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<DIV id=productTitle>Journal of Fish Biology</DIV>
<DIV class=articleDetails xmlns="http://www.w3.org/1999/xhtml"><A
href="http://onlinelibrary.wiley.com/doi/10.1111/jfb.2011.78.issue-4/issuetoc"
shape=rect><FONT color=#000000><SPAN id=volumeNumber>Volume 78</SPAN>, <SPAN
id=issueNumber>Issue 4</SPAN>, </FONT></A><SPAN id=issuePages>pages
1170–1182</SPAN>, <SPAN id=issueDate>April 2011</SPAN></DIV>
<DIV class="citation articleInformationHeader"
xmlns="http://www.w3.org/1999/xhtml">Article first published online: 8 MAR
2011</DIV>
<DIV id=doi>DOI: 10.1111/j.1095-8649.2011.02925.x</DIV>
<DIV class=articleTitle xmlns="http://www.w3.org/1999/xhtml"> </DIV>
<DIV class=articleTitle xmlns="http://www.w3.org/1999/xhtml"><FONT
size=4>Utilization of stomach content DNA to determine diet diversity in
piscivorous fishes</FONT></DIV>
<DIV class="citation articleInformationHeader" id=cr1
xmlns="http://www.w3.org/1999/xhtml">L. Carreon-Martinez<SUP>1</SUP>, T. B.
Johnson<SUP>2</SUP>, S. A. Ludsin<SUP>3</SUP>, D. D. Heath<SUP>1,*</SUP></DIV>
<DIV class="citation articleInformationHeader" id=publishedOnlineDate
xmlns="http://www.w3.org/1999/xhtml"> </DIV>
<DIV id=productTitle><SUP>1</SUP> <EM>Great Lakes Institute for Environmental
Research, University of Windsor, Windsor, Ontario, N9B 3P4
Canada</EM><SUP>2</SUP> <EM>Ontario Ministry of Natural Resources, Glenora
Fisheries Station, R. R. #4, 41 Hatchery Lane, Picton, Ontario, K0K 2T0
Canada</EM><SUP>3</SUP> <EM>Aquatic Ecology Laboratory, Department of Evolution,
Ecology, and Organismal Biology, The Ohio State University, Columbus, OH 43212,
U.S.A. </EM>Correspondence: D. D. Heath, Tel.: +1 519 253 3000, ext. 3762;
email: <!--TODO: clickthrough URL--><A title="Link to email address"
href="mailto:dheath@uwindsor.ca" shape=rect><FONT
color=#007e8a>dheath@uwindsor.ca</FONT></A></DIV>
<UL class="" id=footnotes><FONT color=#007e8a></FONT></UL>
<UL class="" id=footnotes><FONT color=#007e8a></FONT></UL>
<DIV id=publicationHistoryDetails
jQuery1301961922031="10"><STRONG>Summary</STRONG></DIV>
<DIV jQuery1301961922031="10"> </DIV>
<DIV jQuery1301961922031="10">The objective of the study was to validate and
apply DNA-based approaches to describe fish diets. Laboratory experiments were
performed to determine the number of hours after ingestion that DNA could be
reliably isolated from stomach content residues, particularly with small prey
fishes (<EM>c.</EM> 1 cm, <0·75 g). Additionally, experiments were conducted
at different temperatures to resolve temperature effects on digestion rate and
DNA viability. The molecular protocol of cloning and sequencing was then applied
to the analysis of stomach contents of wild fishes collected from the western
basin of Lake Erie, Canada–U.S.A. The results showed that molecular techniques
were more precise than traditional visual inspection and could provide insight
into diet preferences for even highly digested prey that have lost all physical
characteristics.</DIV></FONT></BODY></HTML>