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<DIV><FONT size=2>Acta Zoologica</FONT></DIV>
<DIV id=publishedOnlineDate><FONT size=2>Article first published online: 20 JUN
2013</FONT></DIV>
<DIV id=doi><FONT size=2>DOI: 10.1111/azo.12038</FONT></DIV>
<DIV><FONT size=2></FONT> </DIV></FONT></SPAN>
<DIV class=articleTitle><SPAN class=mainTitle><FONT size=3><STRONG>Histological
and histochemical study of the digestive system of the Argentine anchovy larvae
(<EM>Engraulis anchoita</EM>) at different developmental stages of their
ontogenetic development</STRONG></FONT></SPAN></DIV>
<DIV id=azo12038-cr-0001>Stefanía Cohen<SUP>1,*</SUP>, Marina V.
Diaz<SUP>2</SUP>, Alcira O. Díaz<SUP>1</SUP></DIV>
<DIV><SUP>1</SUP> Departamento de Biología, Laboratorio de Histología e
Histoquímica, Facultad de Ciencias Exactas y Naturales, Instituto de
Investigaciones Marinas y Costeras (IIMyC), CONICET-Universidad Nacional de Mar
del Plata, Mar del Plata, Pcia. de Bs. As, Argentina<SUP>2</SUP> Instituto
Nacional de Investigación y Desarrollo Pesquero (INIDEP), Mar del Plata, Pcia.
de Bs. As, Argentina<SUP>*</SUP>Stefanía Cohen, Departamento de Biología,
Laboratorio de Histología e Histoquímica, Facultad de Ciencias Exactas y
Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC),
CONICET-Universidad Nacional de Mar del Plata. Funes 3250 3° piso, B7602AYJ, Mar
del Plata, Buenos Aires, Argentina. E-mail: <!--TODO: clickthrough URL--><A
title="Link to email address" href="mailto:scohen@mdp.edu.ar" shape=rect><FONT
color=#007e8a>scohen@mdp.edu.ar</FONT></A></DIV>
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<DIV id=publicationHistoryDetails jQuery1372168723159="11">
<H4>Abstract</H4></DIV></DIV></DIV>
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<DIV id=abstract>
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<P>In this work, histological and histochemical features of the larval digestive
system of Argentine anchovy <EM>Engraulis anchoita</EM> were described.
Structural changes during ontogenetic development were also characterized, and
comparisons between the beginning and the end of larval development were made.
Histological sections of larvae were subjected to histochemical and routine
histological techniques to localize and differentiate glycoproteins (GPs). Both
at an early and a late larval stage, the oesophageal goblet cells reacted more
intensely than those of the rest of the digestive tract, and only the oesophagus
exhibited metachromasia with toluidine blue techniques at different pHs, thus
revealing diverse GPs at different concentrations. The GPs histochemical
composition in the intestine varied with the developmental stage and the
intestinal zone. The absence of goblet cells characterized the foregut; however,
they started differentiation at an advanced stage in the midgut. These cells
could be detected in the hindgut both at the beginning and at the end of
development. The attached glands showed a varied glycoprotein composition. The
digestive tract of <EM>E. anchoita</EM> presented a high level of
complexity, related to the multiple functions of mucus in the digestive tract,
such as lubrication, protection, antimicrobial function and ionic and osmotic
regulation.</P></DIV></DIV></DIV></DIV></FONT></DIV></BODY></HTML>