<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META content="text/html; charset=iso-8859-1" http-equiv=Content-Type>
<META name=GENERATOR content="MSHTML 8.00.6001.18702">
<STYLE></STYLE>
</HEAD>
<BODY bgColor=#ffffff><FONT size=2 face=Arial>
<DIV class=articleTitle><SPAN class=mainTitle>
<DIV id=productTitle sizset="5" sizcache="2">Journal of Biogeography</DIV>
<DIV class=articleDetails sizset="5" sizcache="2"><A
href="http://onlinelibrary.wiley.com/doi/10.1111/jbi.2014.41.issue-3/issuetoc"
shape=rect><FONT color=#000000><SPAN id=volumeNumber>Volume 41</SPAN>, <SPAN
id=issueNumber>Issue 3</SPAN>, </FONT></A><SPAN id=issuePages>pages
615–627</SPAN>, <SPAN id=issueDate>March 2014</SPAN></DIV>
<DIV class=articleDetails sizset="5" sizcache="2"><SPAN>
<DIV id=publishedOnlineDate>Article first published online: 12 DEC 2013</DIV>
<DIV id=doi>DOI: 10.1111/jbi.12217</DIV></SPAN></DIV></SPAN></DIV>
<DIV class=articleTitle><SPAN class=mainTitle></SPAN> </DIV>
<DIV class=articleTitle><SPAN class=mainTitle></SPAN> </DIV>
<DIV class=articleTitle><SPAN class=mainTitle><FONT size=5>Phylogeography and
trans-Pacific divergence of the rocky shore gastropod <EM>Nucella
lima</EM></FONT></SPAN></DIV>
<DIV id=jbi12217-cr-0001>L. Nicole Cox<SUP>1</SUP>, Nadezhda I.
Zaslavskaya<SUP>2,3</SUP>, Peter B. Marko<SUP>1,*</SUP></DIV>
<DIV id=publishedOnlineDate><SUP>1</SUP> Department of Biological Sciences,
Clemson University, Clemson, SC, USA<SUP>2</SUP> A. V. Zhirmunsky Institute of
Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok,
Russia<SUP>3</SUP> Far Eastern Federal University, Vladivostok,
Russia<SUP>*</SUP> Correspondence and current address: P. B. Marko E-mail: <!--TODO: clickthrough URL--><A title="Link to email address"
href="mailto:pmarko@hawaii.edu" shape=rect><FONT
color=#007e8a>pmarko@hawaii.edu</FONT></A><BR></DIV>
<DIV>
<DIV id=additionalInformation>
<DIV style="CLEAR: left">
<DIV id=publicationHistoryDetails jQuery1392916165919="11">
<H4>Abstract</H4></DIV></DIV></DIV>
<DIV id=productContent>
<DIV id=fulltext>
<DIV xmlns="http://www.w3.org/1999/xhtml">
<DIV id=abstract>
<DIV id=jbi12217-sec-0001 class=section>
<H4>Aim</H4>
<DIV class=para>
<P>We have used phylogeographical and multilocus coalescent population genetic
methods to reconstruct the Pleistocene biogeographical history of a broadly
distributed north Pacific rocky shore gastropod, <EM>Nucella
lima</EM>.</P></DIV></DIV>
<DIV id=jbi12217-sec-0002 class=section>
<H4>Location</H4>
<DIV class=para>
<P>Northern Pacific rim, from south-eastern Alaska to Hokkaido
Island.</P></DIV></DIV>
<DIV id=jbi12217-sec-0003 class=section>
<H4>Methods</H4>
<DIV class=para>
<P>We gathered DNA sequence data from three loci from <EM>N. lima</EM>,
whose current distribution spans the entire North Pacific rim. We used a
combination of population genetic summary statistics (Tajima's <EM>D</EM>, Fu's
<EM>F</EM><SUB><EM><FONT size=1>S</FONT></EM></SUB> and Φ<SUB><FONT
size=1>ST</FONT></SUB>), isolation-with-migration divergence models, and
extended Bayesian skyline plots to reconstruct the recent biogeographical
history of this species.</P></DIV></DIV>
<DIV id=jbi12217-sec-0004 class=section>
<H4>Results</H4>
<DIV class=para>
<P>The largest values of Φ<SUB><FONT size=1>ST</FONT></SUB> across all loci were
always between eastern and western samples. Population divergence models
indicated no gene flow and mid-Pleistocene divergence times
(> 600 ka) between eastern and western populations. Mitochondrial
DNA diversity in the east was low, and coalescent-based estimates of effective
population size were significantly smaller in the east 20,000 years ago, at
the end of the last glacial period.</P></DIV></DIV>
<DIV id=jbi12217-sec-0005 class=section>
<H4>Main conclusions</H4>
<DIV class=para>
<P>The results are consistent with a hypothesis in which north Pacific
populations were separated into eastern and western refugia by 317 ka with
no gene flow since the split. Eastern populations probably underwent a severe
bottleneck in population size during the last glacial period. The contrasting
demographic histories of eastern and western populations are consistent with the
general palaeobiogeographical pattern of greater climate-related extinction of
marine taxa in the eastern
Pacific.</P></DIV></DIV></DIV></DIV></DIV></DIV></FONT></DIV></BODY></HTML>