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<div class=Section1>
<p class=MsoNormal>Journal of Applied Ichthyology<o:p></o:p></p>
<p class=MsoNormal style='outline: 0px'><b><span style='color:#007E8B'><a
href="http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0426/earlyview"><span
style='font-size:9.0pt;font-family:"Arial","sans-serif";color:#007E8A;
border:none windowtext 1.0pt;padding:0in'>Early View (Online Version of Record
published before inclusion in an issue)</span></a><o:p></o:p></span></b></p>
<p class=MsoNormal><span style='color:black'>Article first published online: 9
MAR 2016<o:p></o:p></span></p>
<p class=MsoNormal style='outline: 0px;word-wrap: break-word' id=doi><span
style='color:black'>DOI: 10.1111/jai.13050<o:p></o:p></span></p>
<h1 style='mso-margin-top-alt:0in;margin-right:24.0pt;margin-bottom:0in;
margin-left:0in;margin-bottom:.0001pt;mso-line-height-alt:7.5pt;vertical-align:
baseline'><span class=maintitle><span style='font-size:10.5pt;font-family:"Arial","sans-serif";
color:black;border:none windowtext 1.0pt;padding:0in'><o:p> </o:p></span></span></h1>
<h1 style='mso-margin-top-alt:0in;margin-right:24.0pt;margin-bottom:0in;
margin-left:0in;margin-bottom:.0001pt;mso-line-height-alt:7.5pt;vertical-align:
baseline'><span class=maintitle><span style='font-size:10.5pt;font-family:"Arial","sans-serif";
color:black;border:none windowtext 1.0pt;padding:0in'><o:p> </o:p></span></span></h1>
<h1 style='mso-margin-top-alt:0in;margin-right:24.0pt;margin-bottom:0in;
margin-left:0in;margin-bottom:.0001pt;mso-line-height-alt:7.5pt;vertical-align:
baseline'><span class=maintitle><span style='font-size:18.0pt;font-family:"Arial","sans-serif";
color:black;border:none windowtext 1.0pt;padding:0in'>A complete protocol for
the preparation of chondrichthyan skeletal specimens</span></span><span
style='font-size:18.0pt;font-family:"Arial","sans-serif";color:black'><o:p></o:p></span></h1>
<p class=MsoNormal style='mso-margin-top-alt:0in;margin-right:3.75pt;
margin-bottom:0in;margin-left:0in;margin-bottom:.0001pt;text-indent:-.25in;
line-height:18.0pt;mso-list:l2 level1 lfo1;vertical-align:baseline'><![if !supportLists]><span
style='font-size:9.0pt;font-family:"Arial","sans-serif";color:black'><span
style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'>
</span></span></span><![endif]><span style='font-size:9.0pt;font-family:"Arial","sans-serif";
color:black'>S. Enault</span><sup><span style='font-size:7.0pt;font-family:
"Arial","sans-serif";color:black;border:none windowtext 1.0pt;padding:0in'>*</span></sup><span
style='font-size:9.0pt;font-family:"Arial","sans-serif";color:black'>,<span
class=apple-converted-space> </span>C. Auclair,<span
class=apple-converted-space> </span>S. Adnet and M. Debiais-Thibaud<o:p></o:p></span></p>
<p class=MsoNormal style='line-height:7.5pt;vertical-align:baseline;outline: 0px'
id=publishedOnlineDate><span style='font-size:7.5pt;font-family:"Arial","sans-serif";
color:#A3A2A2'><o:p> </o:p></span></p>
<p class=MsoNormal style='line-height:7.5pt;vertical-align:baseline'><span
style='font-size:7.5pt;font-family:"Arial","sans-serif";color:black'><o:p> </o:p></span></p>
<p class=MsoNormal style='mso-margin-top-alt:0in;margin-right:6.0pt;margin-bottom:
0in;margin-left:0in;margin-bottom:.0001pt;text-indent:-.25in;mso-line-height-alt:
7.5pt;mso-list:l0 level1 lfo4;background:#007E8B;vertical-align:baseline;
outline: 0px;float:left'><![if !supportLists]><span style='font-size:10.0pt;
font-family:Symbol;color:#757373'><span style='mso-list:Ignore'>·<span
style='font:7.0pt "Times New Roman"'>
</span></span></span><![endif]><b><span style='font-size:8.5pt;font-family:
"Arial","sans-serif";color:white;border:none windowtext 1.0pt;padding:0in'>Abstract</span></b><b><span
style='font-size:8.5pt;font-family:"Arial","sans-serif";color:#757373'><o:p></o:p></span></b></p>
<h4 style='mso-margin-top-alt:0in;margin-right:0in;margin-bottom:.05in;
margin-left:0in;line-height:19.2pt;vertical-align:baseline'><span
style='font-family:"Arial","sans-serif";color:black'>Summary<o:p></o:p></span></h4>
<p style='mso-margin-top-alt:0in;margin-right:0in;margin-bottom:12.0pt;
margin-left:0in;line-height:18.0pt;vertical-align:baseline;outline: 0px'><span
style='font-size:9.0pt;font-family:"Arial","sans-serif";color:black'>The
skeleton of cartilaginous fishes is notoriously difficult to prepare, and the
literature dealing with the subject is extremely rare compared to other
vertebrate groups. As a result, chondrichthyan skeletal specimens held in
museums and other scientific institutions are often limited to isolated sets of
jaws, taxidermised specimens and small individuals preserved whole in fluid.
However, skeletal characters are heavily relied upon in several taxonomical and
phylogenetical analyses, highlighting the importance of properly prepared
skeletal specimens in research collections. The relevant literature is briefly
reviewed herein, with a proposal for a new and simple protocol involving
limited and easily obtainable chemicals, for the quick preparation of clean and
durable chondrichthyan skeletal specimens. Various features of the
chondrichthyan skeleton are also discussed, along with the microstructural
effects of the chemicals involved in the protocol.<o:p></o:p></span></p>
<p class=MsoNormal><o:p> </o:p></p>
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