[OANNES Foro] Utilization of stomach content DNA to determine diet diversity in piscivorous fishes

[Mario Cabrejos]

Journal of Fish Biology
Article first published online: 8 MAR 2011
DOI: 10.1111/j.1095-8649.2011.02925.x

Utilization of stomach content DNA to determine diet diversity in piscivorous fishes
L. Carreon-Martinez1, T. B. Johnson2, S. A. Ludsin3, D. D. Heath1,*
1 Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4 Canada2 Ontario Ministry of Natural Resources, Glenora Fisheries Station, R. R. #4, 41 Hatchery Lane, Picton, Ontario, K0K 2T0 Canada3 Aquatic Ecology Laboratory, Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, OH 43212, U.S.A. *Correspondence: D. D. Heath,  +1 519 253 3000, ext. 3762; email: dheath en uwindsor.ca

Summary
The objective of the study was to validate and apply DNA-based approaches to describe fish diets. Laboratory experiments were performed to determine the number of hours after ingestion that DNA could be reliably isolated from stomach content residues, particularly with small prey fishes (c. 1 cm, <0·75 g). Additionally, experiments were conducted at different temperatures to resolve temperature effects on digestion rate and DNA viability. The molecular protocol of cloning and sequencing was then applied to the analysis of stomach contents of wild fishes collected from the western basin of Lake Erie, Canada-U.S.A. The results showed that molecular techniques were more precise than traditional visual inspection and could provide insight into diet preferences for even highly digested prey that have lost all physical characteristics.
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